Biological Sciences Division
Published August 15, 2017 | Version v1
Journal article Open

Deconstruction of the beaten path-sidestep interaction network provides insights into neuromuscular system development

Creators

  • 1. California Institute of Technology
  • 2. University of Chicago
  • 3. Ellipsis Health
  • 4. Trinity College Dublin
  • 5. Stanford University

Description

An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we 'deorphanized' four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons.

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Additional details

Identifiers

DOI
10.7554/eLife.28111
Other
oai:uchicago.tind.io:9859

Funding

National Institutes of Health
RO1 NS097161
The Klingenstein-Simons Fellowship Awards in the Neurosciences
Science Foundation Ireland
07/IN.1/B913
Science Foundation Ireland
08/RFP/NSC1617
National Institutes of Health
R37 NS28182

UChicago Information

Division(s)
Biological Sciences Division
Department(s)
Biochemistry and Molecular Biology