Centers and Institutes
Published March 29, 2021 | Version v1
Journal article Open

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

Creators

  • 1. The Hebrew University of Jerusalem
  • 2. Heinrich-Heine-Universität
  • 3. Hasselt University
  • 4. University of Sheffield
  • 5. Aarhus University
  • 6. St. Jude Children's Research Hospital
  • 7. University of Applied Science Mittweida
  • 8. National Institute of Diabetes and Digestive and Kidney Diseases
  • 9. Ludwig-Maximilians-Universität München
  • 10. The Scripps Research Institute
  • 11. University of Cincinnati
  • 12. University of Chicago
  • 13. Columbia University
  • 14. Johns Hopkins University
  • 15. Weizmann Institute of Science
  • 16. University of Copenhagen
  • 17. Seoul National University

Description

Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB-Dev. This position paper describes the current 'state of the art' from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of 'soft recommendations' about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. To make further progress, we strongly encourage 'open science' practices.

Notes

Due to the large number of authors, only the first 20 and the University of Chicago authors are included on the above author list. Please download the article for the complete list of authors.

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Additional details

Identifiers

DOI
10.7554/eLife.60416
Other
oai:uchicago.tind.io:10858

Funding

National Institutes of Health
GM130942
National Institutes of Health
GM095904
National Institutes of Health
GM079238
National Institutes of Health
GM098859
National Institutes of Health
GM084288
National Institutes of Health
GM137608
National Institutes of Health
GM112882
National Institutes of Health
GM130793
National Institutes of Health
GM140272
National Institutes of Health
GM130375
National Institutes of Health
128185
National Institute of Diabetes and Digestive and Kidney Diseases
National Science Foundation
1842951
National Science Foundation
2004016
Human Frontier Science Program
RGP0061/2019
European Research Council
638536
European Research Council
SMPFv2.0
European Research Council
671208
European Research Council
819299
Deutsche Forschungsgemeinschaft
GRK2062
Deutsche Forschungsgemeinschaft
SFB863
Deutsche Forschungsgemeinschaft
PL696/4-1
Deutsche Forschungsgemeinschaft
SPP2191 402723784
Deutsche Forschungsgemeinschaft
SFB 1129 240245660
Deutsche Forschungsgemeinschaft
SE 1195/21-1
Deutsche Forschungsgemeinschaft
SPP 2191
Deutsche Forschungsgemeinschaft
project ID 390939984
Wellcome Trust
110164/Z/15/Z
Swiss National Science Foundation
Biotechnology and Biological Sciences Research Council
BB/S008896/1
Biotechnology and Biological Sciences Research Council
BB/T008032/1
Royal Society
RGS\R2\180405
Royal Society
DKR00620
Royal Society
RGF\R1\180054
Agence Nationale de la Recherche
ANR-17-CE09-0026-02
Agence Nationale de la Recherche
ANR-18-CE11-0004-02
Agence Nationale de la Recherche
ANR-19-CE44-0009-02
Israel Science Foundation
1250/19
Israel Science Foundation
3565/20
National Research Foundation of Korea
2019R1A2C2090896
National Research Foundation of Korea
NRF-2019R1A2C2005209
National Research Foundation of Korea
NRF-2019R1A2C1089808
Independent Fund Denmark
6110-00623B
National Key Research and Development Program of China
2018YFA0507700
UHasselt
BOF fund
Deutsche Forschungsgemeinschaft
SFB1035
Milner Fund
KU Leuven
Special Research Fund
Carlsbergfondet
CF16-0797
Villum Fonden
18333
Novo Nordisk
NNF14CC0001
Ludwig-Maximilians-Universität München
Carolina Cancer Center of Nanotechnology Excellence
University of Melbourne
Searle Scholars Program
University of Zurich
EPSRC

UChicago Information

Division(s)
Biological Sciences Division
Department(s)
Biochemistry and Molecular Biology
Center(s) or Institute(s)
Institute for Biophysical Dynamics